Plasmid

Part:BBa_K5048022:Design

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-14)


plasmid QEP_6xhis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5668
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5668
    Illegal NotI site found at 5693
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5668
    Illegal BglII site found at 4964
    Illegal BamHI site found at 5662
    Illegal XhoI site found at 5702
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5668
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5668
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
    Illegal AgeI site found at 5409
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2263


Design Notes

We add enterokinase site between Lpp'OmpA and small peptides in order to verify the enzymatic cleavage.



Source

The insertion fragment is complete synthesis . The backbone is purchased from Genscript.


References